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KMID : 0380919960250010143
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Journal of the Korean Society of Food Nutrition
1996 Volume.25 No. 1 p.143 ~ p.149
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Studies on the Electrofusion between Lactobacillus acidophilus 88 and Lactobacillus bulgaricus IFO 13953
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Jo Young-Bae
Kim Hye-Jeong
Kim Sung-Koo
Baik Hyung-Suk
Jun Hong-Ki
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Abstract
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The optimal conditions for eletrofusion between the cells of streptomycin(2㎎/ml) and lincomycin(5㎍/ml) resistant Lactobacillus bulgaricus IFO 13953 and of kanamycin(600㎍/ml) resistant Lactobacillus acidophilus 88 were investigated. The highest numbers of electrofusant were obtained at a capacitance value of 100msec(2,300uF), a field strength of 500V / ㎝ and at a pulse controller setting of 49ohms. When various divalent cations were added to the electroporation buffer, the electrofusion yield was slightly increased by the addition of 1mM Mg^(2+). Electrofusants were very efficiently obtained by addition of 30% polyethylene glycol in electroporation buffer. Fusion yield of electroporation was better than that of polyethylene glycol mediated chemical induction method.
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KEYWORD
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Lactbacillus acidophilus, Lactobacillus bulgaricus, electrofusion
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